COL1A1 Kit ELISA

N° du produit ABIN5653098

Détail du produit COL1A1 Kit ELISA

Antigène: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 1 (COL1a1). No significant cross-reactivity or interference between Collagen Type I Alpha 1 (COL1a1) and analogues was observed.

Sensibilité: 12.1 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 1 (COL1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 1 (COL1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 1 (COL1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 1 (COL1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Autre désignation: Collagen Type I Alpha 1 (COL1A1 Produits)

Synonymes: OI4 Kit ELISA, Col1a-1 Kit ELISA, Cola-1 Kit ELISA, Cola1 Kit ELISA, Mov-13 Kit ELISA, Mov13 Kit ELISA, COLIA1 Kit ELISA, col1a1 Kit ELISA, MGC52532 Kit ELISA, COL1A1 Kit ELISA, COL1A2 Kit ELISA, alpha1(I) Kit ELISA, cb21 Kit ELISA, sb:cb21 Kit ELISA, sb:cb384 Kit ELISA, wu:fa99c12 Kit ELISA, wu:fb02c06 Kit ELISA, alpha3(I) Kit ELISA, col1a3 Kit ELISA, hm:zeh0348 Kit ELISA, wu:fa95h05 Kit ELISA, wu:fd02a10 Kit ELISA, wu:fj59a10 Kit ELISA, collagen type I alpha 1 chain Kit ELISA, collagen, type I, alpha 1 Kit ELISA, collagen, type I, alpha 1 S homeolog Kit ELISA, collagen alpha-1(I) chain Kit ELISA, collagen type I alpha 2 chain Kit ELISA, collagen, type I, alpha 1a Kit ELISA, collagen 1a1 Kit ELISA, collagen, type I, alpha 1b Kit ELISA, COL1A1 Kit ELISA, Col1a1 Kit ELISA, col1a1.S Kit ELISA, LOC397571 Kit ELISA, COL1A2 Kit ELISA, col1a1a Kit ELISA, col1a1 Kit ELISA, col1a1b Kit ELISA

Sujet:

Gene Name: Collagen Type I Alpha 1

Gene Aliases: COL-1A1, COL1-A1, COL1A-1, OI4, Collagen Alpha-1(I)chain

ID gène: 5300

UniProt: Q13526

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding