SERPING1 Kit CLIA

N° du produit ABIN5653189

Détail du produit SERPING1 Kit CLIA

Antigène: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 0.14 ng/mL - 100 ng/mL

Seuil minimal de détection: 0.14 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Complement 1 Inhibitor (C1INH). No significant cross-reactivity or interference between Complement 1 Inhibitor (C1INH) and analogues was observed.

Sensibilité: 0.06 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement 1 Inhibitor (C1INH) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Autre désignation: Complement 1 Inhibitor (SERPING1 Produits)

Synonymes: fb64f02 Kit CLIA, im:7159527 Kit CLIA, si:dkeyp-110g5.2 Kit CLIA, wu:fb60c09 Kit CLIA, wu:fb62a08 Kit CLIA, wu:fb64f02 Kit CLIA, LOC100136072 Kit CLIA, C1-INH Kit CLIA, C1INH Kit CLIA, C1nh Kit CLIA, C1IN Kit CLIA, C1NH Kit CLIA, HAE1 Kit CLIA, HAE2 Kit CLIA, serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 Kit CLIA, C1 inhibitor Kit CLIA, serpin family G member 1 Kit CLIA, serine (or cysteine) peptidase inhibitor, clade G, member 1 Kit CLIA, serping1 Kit CLIA, LOC100136072 Kit CLIA, SERPING1 Kit CLIA, Serping1 Kit CLIA

Sujet:

Gene Name: Complement 1 Inhibitor

Gene Aliases: SERPING1, C1IN, C1-INH, C1NH, HAE1, HAE2, Serpin Peptidase Inhibitor Clade G Member 1, Angioedema Hereditary, C1 Inhibitor, C1 Esterase Inhibitor

ID gène: 710

UniProt: P05155

Pathways: Système du Complément