ERK1 Kit ELISA

N° du produit ABIN5654027

Détail du produit ERK1 Kit ELISA

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Extracellular Signal Regulated Kinase 1 (ERK1). No significant cross-reactivity or interference between Extracellular Signal Regulated Kinase 1 (ERK1) and analogues was observed.

Sensibilité: 0.118 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Signal Regulated Kinase 1 (ERK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Signal Regulated Kinase 1 (ERK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Signal Regulated Kinase 1 (ERK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Extracellular Signal Regulated Kinase 1 (ERK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Extracellular Signal Regulated Kinase 1 (ERK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Extracellular Signal Regulated Kinase 1 (ERK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Autre désignation: Extracellular Signal Regulated Kinase 1 (MAPK3 Produits)

Synonymes: ERK-1 Kit ELISA, ERK1 Kit ELISA, ERT2 Kit ELISA, HS44KDAP Kit ELISA, HUMKER1A Kit ELISA, P44ERK1 Kit ELISA, P44MAPK Kit ELISA, PRKM3 Kit ELISA, p44-ERK1 Kit ELISA, p44-MAPK Kit ELISA, Erk-1 Kit ELISA, Erk1 Kit ELISA, Ert2 Kit ELISA, Esrk1 Kit ELISA, Mnk1 Kit ELISA, Mtap2k Kit ELISA, Prkm3 Kit ELISA, p44 Kit ELISA, p44erk1 Kit ELISA, p44mapk Kit ELISA, ERK3 Kit ELISA, ERK6 Kit ELISA, P38GAMMA Kit ELISA, PRKM12 Kit ELISA, SAPK-3 Kit ELISA, SAPK3 Kit ELISA, fi06b09 Kit ELISA, wu:fi06b09 Kit ELISA, zERK1 Kit ELISA, Tb08.10J17.940 Kit ELISA, MAPK1 Kit ELISA, MNK1 Kit ELISA, AW123708 Kit ELISA, Erk6 Kit ELISA, P38gamma Kit ELISA, Prkm12 Kit ELISA, Sapk3 Kit ELISA, ATMAPK3 Kit ELISA, ATMPK3 Kit ELISA, T6D9.4 Kit ELISA, mitogen-activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase 12 Kit ELISA, mitogen activated protein kinase 3 Kit ELISA, mitogen-activated serine/threonine-protein kinase Kit ELISA, MAPK3 Kit ELISA, Mapk3 Kit ELISA, MAPK12 Kit ELISA, mapk3 Kit ELISA, Tc00.1047053509475.10 Kit ELISA, Tb927.8.3550 Kit ELISA, Mapk12 Kit ELISA, CEK1 Kit ELISA, MPK3 Kit ELISA

Sujet:

Gene Name: Extracellular Signal Regulated Kinase 1

Gene Aliases: HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAPK3, ERT2, Mitogen-Activated Protein Kinase 3, Insulin-stimulated MAP2 kinase, Microtubule-associated protein 2 kinase

ID gène: 5595

UniProt: P27361

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins