FYN Kit ELISA

N° du produit ABIN5654449

Détail du produit FYN Kit ELISA

Antigène: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of FYN Oncogene Related To SRC/FGR/YES (FYN). No significant cross-reactivity or interference between FYN Oncogene Related To SRC/FGR/YES (FYN) and analogues was observed.

Sensibilité: 0.06 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FYN Oncogene Related To SRC/FGR/YES (FYN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FYN Oncogene Related To SRC/FGR/YES (FYN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FYN Oncogene Related To SRC/FGR/YES (FYN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FYN Oncogene Related To SRC/FGR/YES (FYN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FYN Oncogene Related To SRC/FGR/YES (FYN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FYN Oncogene Related To SRC/FGR/YES (FYN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Autre désignation: FYN Oncogene Related To SRC/FGR/YES (FYN Produits)

Synonymes: im:6908851 Kit ELISA, si:dkey-119m7.5 Kit ELISA, zgc:109996 Kit ELISA, c-fyn Kit ELISA, cfyn-a Kit ELISA, SLK Kit ELISA, SYN Kit ELISA, p59-FYN Kit ELISA, PKC Kit ELISA, fyn Kit ELISA, zgc:86720 Kit ELISA, AI448320 Kit ELISA, AW552119 Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase S homeolog Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase b Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase L homeolog Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase a Kit ELISA, Fyn proto-oncogene Kit ELISA, FYN Kit ELISA, fyn.S Kit ELISA, fynb Kit ELISA, fyn.L Kit ELISA, Fyn Kit ELISA, fyna Kit ELISA

Sujet:

Gene Name: FYN Oncogene Related To SRC/FGR/YES

Gene Aliases: SLK, SYN, c-Fyn, Src-like kinase

ID gène: 2534

UniProt: P06241

Pathways: Signalistation JAK/STAT, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling