SLC2A3 Kit ELISA

N° du produit ABIN5654622

Détail du produit SLC2A3 Kit ELISA

Antigène: SLC2A3 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 3 (SLC2A3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glucose Transporter 3 (GLUT3). No significant cross-reactivity or interference between Glucose Transporter 3 (GLUT3) and analogues was observed.

Sensibilité: 0.29 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose Transporter 3 (GLUT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose Transporter 3 (GLUT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose Transporter 3 (GLUT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose Transporter 3 (GLUT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose Transporter 3 (GLUT3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose Transporter 3 (GLUT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SLC2A3 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 3 (SLC2A3))

Autre désignation: Glucose Transporter 3 (SLC2A3 Produits)

Synonymes: slc2a3 Kit ELISA, wu:fb92c06 Kit ELISA, zgc:92476 Kit ELISA, SLC2A3 Kit ELISA, GLUT3 Kit ELISA, AA408729 Kit ELISA, AL023014 Kit ELISA, AL024341 Kit ELISA, AU040424 Kit ELISA, C78366 Kit ELISA, Glut-3 Kit ELISA, Glut3 Kit ELISA, CEF-GT3 Kit ELISA, GLUT-3 Kit ELISA, SLC2A14 Kit ELISA, solute carrier family 2 (facilitated glucose transporter), member 3a Kit ELISA, solute carrier family 2 member 3 Kit ELISA, solute carrier family 2 (facilitated glucose transporter), member 3 Kit ELISA, solute carrier family 2 member 14 Kit ELISA, solute carrier family 2, facilitated glucose transporter member 3 Kit ELISA, slc2a3a Kit ELISA, SLC2A3 Kit ELISA, Slc2a3 Kit ELISA, SLC2A14 Kit ELISA, LOC100125981 Kit ELISA

Sujet:

Gene Name: Glucose Transporter 3

Gene Aliases: SLC2A3, Solute Carrier Family 2(Facilitated Glucose Transporter)Member 3, Glucose transporter type 3, brain

ID gène: 6515

UniProt: P11169

Pathways: L'effet Warburg