KPNA2 Kit ELISA

N° du produit ABIN5656172

Détail du produit KPNA2 Kit ELISA

Antigène: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125.00 pg/mL - 8000 pg/mL

Seuil minimal de détection: 125.00 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Karyopherin Alpha 2 (KPNa2). No significant cross-reactivity or interference between Karyopherin Alpha 2 (KPNa2) and analogues was observed.

Sensibilité: 53 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Alpha 2 (KPNa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Karyopherin Alpha 2 (KPNa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Karyopherin Alpha 2 (KPNa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Karyopherin Alpha 2 (KPNa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Karyopherin Alpha 2 (KPNa2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Karyopherin Alpha 2 (KPNa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Autre désignation: Karyopherin Alpha 2 (KPNA2 Produits)

Synonymes: IPOA1 Kit ELISA, QIP2 Kit ELISA, RCH1 Kit ELISA, SRP1alpha Kit ELISA, ima2 Kit ELISA, importin Kit ELISA, 2410044B12Rik Kit ELISA, PTAC58 Kit ELISA, Rch1 Kit ELISA, ipoa1 Kit ELISA, kpna2 Kit ELISA, qip2 Kit ELISA, rch1 Kit ELISA, srp1alpha Kit ELISA, hm:zeh0389 Kit ELISA, hm:zeh0389r Kit ELISA, zgc:113902 Kit ELISA, zgc:86945 Kit ELISA, karyopherin subunit alpha 2 Kit ELISA, importin subunit alpha-2 Kit ELISA, Importin subunit alpha-2 Kit ELISA, karyopherin (importin) alpha 2 Kit ELISA, karyopherin alpha-2 subunit like L homeolog Kit ELISA, karyopherin alpha 2 (RAG cohort 1, importin alpha 1) Kit ELISA, KPNA2 Kit ELISA, EDI_246290 Kit ELISA, EDI_335040 Kit ELISA, ima2 Kit ELISA, Kpna2 Kit ELISA, kpna2.L Kit ELISA, kpna2 Kit ELISA

Sujet:

Gene Name: Karyopherin Alpha 2

Gene Aliases: IPOA1, QIP2, RCH1, SRP1alpha, RAG Cohort 1, Importin Alpha 1, RAG cohort protein 1, SRP1-alpha

ID gène: 3838

UniProt: P52292

Pathways: M Phase, Protein targeting to Nucleus