Glucocorticoid Receptor Kit ELISA

N° du produit ABIN5657465

Détail du produit Glucocorticoid Receptor Kit ELISA

Antigène: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). No significant cross-reactivity or interference between Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Autre désignation: Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1 Produits)

Synonymes: GR Kit ELISA, gcr Kit ELISA, grl Kit ELISA, xGR Kit ELISA, gccr Kit ELISA, gr Kit ELISA, zgc:113038 Kit ELISA, NR3C1 Kit ELISA, GCL Kit ELISA, LOC100302444 Kit ELISA, Gcr Kit ELISA, Grl Kit ELISA, Grl-1 Kit ELISA, Grl1 Kit ELISA, GRL Kit ELISA, GCCR Kit ELISA, GCR Kit ELISA, NO Kit ELISA, GR-A Kit ELISA, nr3c1 Kit ELISA, nuclear receptor subfamily 3 group C member 1 Kit ELISA, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) Kit ELISA, nuclear receptor subfamily 3, group C, member 1 Kit ELISA, glucocorticoid receptor Kit ELISA, NR3C1 Kit ELISA, nr3c1 Kit ELISA, LOC100302444 Kit ELISA, Nr3c1 Kit ELISA

Sujet:

Gene Name: Nuclear Receptor Subfamily 3, Group C, Member 1

Gene Aliases: GR, GCCR, GCR, GR, GRL, NR3-C1, Glucocorticoid Receptor

ID gène: 24413

UniProt: P06536

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process