POLD1 Kit ELISA

N° du produit ABIN5657980

Détail du produit POLD1 Kit ELISA

Antigène: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Polymerase DNA Directed Delta 1 (POLd). No significant cross-reactivity or interference between Polymerase DNA Directed Delta 1 (POLd) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymerase DNA Directed Delta 1 (POLd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polymerase DNA Directed Delta 1 (POLd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymerase DNA Directed Delta 1 (POLd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polymerase DNA Directed Delta 1 (POLd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polymerase DNA Directed Delta 1 (POLd) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polymerase DNA Directed Delta 1 (POLd) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Autre désignation: Polymerase DNA Directed Delta 1 (POLD1 Produits)

Synonymes: CDC2 Kit ELISA, CRCS10 Kit ELISA, POLD Kit ELISA, 5949 Kit ELISA, CAA61369 Kit ELISA, CG5949 Kit ELISA, DNA Pol Delta Kit ELISA, Dm Poldelta Kit ELISA, Dmel\\CG5949 Kit ELISA, Poldelta Kit ELISA, dnapol delta Kit ELISA, l(3)72Ac Kit ELISA, cdc2 Kit ELISA, pold Kit ELISA, 125kDa Kit ELISA, Pol delta Kit ELISA, fla Kit ELISA, im:7139126 Kit ELISA, zgc:152688 Kit ELISA, DNA polymerase delta 1, catalytic subunit Kit ELISA, DNA-polymerase-delta Kit ELISA, polymerase (DNA directed), delta 1, catalytic subunit Kit ELISA, DNA polymerase delta catalytic subunit Kit ELISA, DNA polymerase delta Kit ELISA, POLD1 Kit ELISA, DNApol-delta Kit ELISA, pold1 Kit ELISA, LOC100456571 Kit ELISA, Pold1 Kit ELISA

Sujet:

Gene Name: Polymerase DNA Directed Delta 1

Gene Aliases: CDC2, POLD1, DNA polymerase subunit delta p125

ID gène: 5424

UniProt: P28340

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Chromatin Binding, Synthesis of DNA