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TRAF6 Kit ELISA

TRAF6 Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5659338
  • Antigène Voir toutes TRAF6 Kits ELISA
    TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))
    Reactivité
    • 3
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of TNF Receptor Associated Factor 6 (TRAF6). No significant cross-reactivity or interference between TNF Receptor Associated Factor 6 (TRAF6) and analogues was observed.
    Sensibilité
    0.053 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor Associated Factor 6 (TRAF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNF Receptor Associated Factor 6 (TRAF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor Associated Factor 6 (TRAF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNF Receptor Associated Factor 6 (TRAF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNF Receptor Associated Factor 6 (TRAF6) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNF Receptor Associated Factor 6 (TRAF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes TRAF6 Kits ELISA
    TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))
    Autre désignation
    TNF Receptor Associated Factor 6 (TRAF6 Produits)
    Synonymes
    MGC:3310 Kit ELISA, RNF85 Kit ELISA, 2310003F17Rik Kit ELISA, AI851288 Kit ELISA, C630032O20Rik Kit ELISA, rnf85 Kit ELISA, traf6 Kit ELISA, xtraf6 Kit ELISA, CG10961 Kit ELISA, DTRAF2 Kit ELISA, Dmel\\CG10961 Kit ELISA, TRAF Kit ELISA, TRAF2 Kit ELISA, TRAF6 Kit ELISA, Traf2 Kit ELISA, dTRAF2 Kit ELISA, dTRAF6 Kit ELISA, dTraf2 Kit ELISA, dtraf2 Kit ELISA, GB10539 Kit ELISA, fi15f02 Kit ELISA, wu:fi15f02 Kit ELISA, zgc:63704 Kit ELISA, TNF receptor associated factor 6 Kit ELISA, TNF receptor-associated factor 6 Kit ELISA, TNF receptor associated factor 6 L homeolog Kit ELISA, TNF-receptor-associated factor 6 Kit ELISA, TNF receptor associated factor 6 S homeolog Kit ELISA, TRAF6 Kit ELISA, Traf6 Kit ELISA, traf6.L Kit ELISA, traf6 Kit ELISA, LOC551819 Kit ELISA, traf6.S Kit ELISA
    Sujet

    Gene Name: TNF Receptor Associated Factor 6

    Gene Aliases: RNF85, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer

    ID gène
    7189
    UniProt
    Q9Y4K3
    Pathways
    Signalisation NF-kappaB, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway
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