XRCC6 Kit CLIA

N° du produit ABIN5660055

Détail du produit XRCC6 Kit CLIA

Antigène: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 312.5 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312.5 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of X-Ray Repair Cross Complementing 6 (XRCC6). No significant cross-reactivity or interference between X-Ray Repair Cross Complementing 6 (XRCC6) and analogues was observed.

Sensibilité: 11.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the X-Ray Repair Cross Complementing 6 (XRCC6) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 6 (XRCC6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 6 (XRCC6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Autre désignation: X-Ray Repair Cross Complementing 6 (XRCC6 Produits)

Synonymes: CTC75 Kit CLIA, CTCBF Kit CLIA, G22P1 Kit CLIA, KU70 Kit CLIA, ML8 Kit CLIA, TLAA Kit CLIA, 70kDa Kit CLIA, G22p1 Kit CLIA, Ku70 Kit CLIA, Kup70 Kit CLIA, X-ray repair cross complementing 6 Kit CLIA, ATP-dependent DNA helicase II, 70 kDa subunit Kit CLIA, X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog Kit CLIA, X-ray repair complementing defective repair in Chinese hamster cells 6 Kit CLIA, XRCC6 Kit CLIA, Bm1_41430 Kit CLIA, xrcc6.L Kit CLIA, Xrcc6 Kit CLIA

Sujet:

Gene Name: X-Ray Repair Cross Complementing 6

Gene Aliases: Ku70, CTC75, CTCBF, G22P1, ML8, TLAA, Lupus Ku Autoantigen Protein P70, ATP-Dependent DNA Helicase 2 Subunit 1, CTC box-binding factor 75 kDa, Thyroid-lupus autoantigen

Pathways: Réparation de l'ADN