Glucocorticoid Receptor Kit ELISA
NR3C1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690884
-
-
Antigène
Voir toutes Glucocorticoid Receptor (NR3C1) Kits ELISA
-
Glucocorticoid Receptor (NR3C1)
(Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))
-
Reactivité
-
Humain
-
Méthode de détection
-
Colorimetric
-
Type de méthode
-
Sandwich ELISA
-
Application
-
ELISA
-
Fonction
-
Human GR/NR3C1 ELISA Kit.
-
Type d'échantillon
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
Specificité
-
This ELISA antibody pair recognizes Human GR.
-
Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
Featured
-
Discover our best selling NR3C1 Kit ELISA
-
Top Product
-
Discover our top product NR3C1 Kit ELISA
-
-
-
Plaque
-
Pre-coated
-
Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
Restrictions
-
For Research Use only
-
-
Stock
-
4 °C
-
Date de péremption
-
6 months
-
-
Antigène
Voir toutes Glucocorticoid Receptor (NR3C1) Kits ELISA
-
Glucocorticoid Receptor (NR3C1)
(Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))
-
Autre désignation
-
GR (NR3C1 (NR3C1 Produits)
-
Synonymes
-
GR Kit ELISA, gcr Kit ELISA, grl Kit ELISA, xGR Kit ELISA, gccr Kit ELISA, gr Kit ELISA, zgc:113038 Kit ELISA, NR3C1 Kit ELISA, GCL Kit ELISA, LOC100302444 Kit ELISA, Gcr Kit ELISA, Grl Kit ELISA, Grl-1 Kit ELISA, Grl1 Kit ELISA, GRL Kit ELISA, GCCR Kit ELISA, GCR Kit ELISA, NO Kit ELISA, GR-A Kit ELISA, nr3c1 Kit ELISA, nuclear receptor subfamily 3 group C member 1 Kit ELISA, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) Kit ELISA, nuclear receptor subfamily 3, group C, member 1 Kit ELISA, glucocorticoid receptor Kit ELISA, NR3C1 Kit ELISA, nr3c1 Kit ELISA, LOC100302444 Kit ELISA, Nr3c1 Kit ELISA
-
ID gène
-
2908
-
UniProt
-
P04150
-
Pathways
-
Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process
-