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HGF Kit ELISA
HGF
Reactivité: Humain
Colorimetric
Sandwich ELISA
3-2000 pg/mL
Cell Lysate, Tissue Lysate
Détail du produit HGF Kit ELISA
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Antigène
Voir toutes HGF Kits ELISA
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Reactivité
Toutes les réactivités sur HGF Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
3-2000 pg/mL
Seuil minimal de détection
3 pg/mL
Application
ELISA
Fonction
Human HGF ELISA Kit for cell and tissue lysate samples.
Type d'échantillon
Tissue Lysate, Cell Lysate
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes human HGF.
Sensibilité
3 pg/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Cell lysate buffer
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes HGF Kits ELISA
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Autre désignation
HGF (HGF Produits )
Synonymes
DFNB39 Kit ELISA, F-TCF Kit ELISA, HGFB Kit ELISA, HPTA Kit ELISA, SF Kit ELISA, HGF Kit ELISA, C230052L06Rik Kit ELISA, HGF/SF Kit ELISA, NK1 Kit ELISA, NK2 Kit ELISA, SF/HGF Kit ELISA, hepatocyte growth factor Kit ELISA, HGF Kit ELISA, Hgf Kit ELISA
Sujet
Gene Names: HGF HPTA Protein names: Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain]
ID gène
3082
UniProt
P14210
Pathways
Signalisation RTK , Carbohydrate Homeostasis , Glycosaminoglycan Metabolic Process , Synaptic Membrane , Signaling of Hepatocyte Growth Factor Receptor
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