TNFRSF11A Kit ELISA

N° du produit ABIN6719860

Détail du produit TNFRSF11A Kit ELISA

Antigène: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD265, FEO, LOH18CR1, Loss of heterozygosity 18 chromosomal region 1, ODFR, OFE, OPTB7, Osteoclast differentiation factor receptor, OSTS, PDB2, Receptor activator of NF-KB, Receptor activator of nuclear factor kappa B, TNFRSF11A, TNR11_HUMAN, TRANCER, Tumor necrosis factor receptor superfamily member 11A, Tumor necrosis factor receptor superfamily member 11a activator of NFKB, Tumor necrosis factor receptor superfamily member 11a NFKB activator

Background: Receptor Activator of Nuclear Factor kB (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.

Gene Name: TNFRSF11A

Production: Natural and recombinant rat RANK. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q30-P213

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat RANK standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. It is recommended that each rat RANK standard solution and each sample be measured in duplicate.

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Autre désignation: RANK (TNFRSF11A Produits)

Synonymes: RANK Kit ELISA, RGD1563614 Kit ELISA, TNFRSF11A Kit ELISA, Ly109 Kit ELISA, ODFR Kit ELISA, OFE Kit ELISA, Rank Kit ELISA, TRANCE-R Kit ELISA, mRANK Kit ELISA, CD265 Kit ELISA, FEO Kit ELISA, LOH18CR1 Kit ELISA, OPTB7 Kit ELISA, OSTS Kit ELISA, PDB2 Kit ELISA, TRANCER Kit ELISA, TNF receptor superfamily member 11A Kit ELISA, TNF receptor superfamily member 11a Kit ELISA, tumor necrosis factor receptor superfamily, member 11a, NFKB activator Kit ELISA, Tnfrsf11a Kit ELISA, TNFRSF11A Kit ELISA

ID gène: 498206

NCBI Accession: NP_001258164

UniProt: F1M8Z6

Pathways: Signalisation NF-kappaB