TRAIL Kit ELISA

N° du produit ABIN6719880

Détail du produit TRAIL Kit ELISA

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Apo 2 ligand, APO2L, CD253, Protein TRAIL, TL2, TNF related apoptosis inducing ligand, Tumor necrosis factor ligand superfamily member 10

Background: In the field of cell biology, TNF-related apoptosis-inducing ligand(TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253(cluster of differentiation 253).Tumor necrosis factor(TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4(TRAIL-RI) and DR5(TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain(DcR1) or contain a truncated death domain(DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28.

Gene Name: TNFSF10

Production: Natural and recombinant human TRAIL. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: T95-G281

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TRAIL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or saliva to each empty well. It is recommended that each human TRAIL standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Autre désignation: TRAIL (TNFSF10 Produits)

Synonymes: APO2L Kit ELISA, Apo-2L Kit ELISA, CD253 Kit ELISA, TL2 Kit ELISA, TRAIL Kit ELISA, A330042I21Rik Kit ELISA, AI448571 Kit ELISA, APO-2L Kit ELISA, Ly81 Kit ELISA, Trail Kit ELISA, tl2 Kit ELISA, apo2l Kit ELISA, cd253 Kit ELISA, trail Kit ELISA, apo-2l Kit ELISA, trail1 Kit ELISA, xtrail1 Kit ELISA, tnfsf10l2 Kit ELISA, zgc:92320 Kit ELISA, TNLG6A Kit ELISA, TNF superfamily member 10 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 10 Kit ELISA, tumor necrosis factor superfamily member 10 Kit ELISA, TNF superfamily member 10 S homeolog Kit ELISA, TNFSF10 Kit ELISA, Tnfsf10 Kit ELISA, tnfsf10.S Kit ELISA, tnfsf10 Kit ELISA

ID gène: 8743

NCBI Accession: NP_001177871

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity