TRAIL Kit ELISA

N° du produit ABIN921077

Détail du produit TRAIL Kit ELISA

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Épitope: AA 95-281

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TRAIL

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Saliva

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO,T95-G281

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: T95-G281

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the tumor necrosis factor family.

Tissue Specificity: Widespread, most predominant in spleen, lung and prostate.

Plaque: Pre-coated

Protocole: human TRAIL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TRAIL has been precoated onto 96-well plates. Standards (NSO,T95-G281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TRAIL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TRAIL amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TRAIL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TRAIL standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 102, Standard deviation: 4.59, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 368, Standard deviation: 12.88, CV(%): 3.5
• Sample 3: n=16, Mean(pg/ml): 637, Standard deviation: 39.5, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 167, Standard deviation: 13.36, CV(%): 8
• Sample 2: n=24, Mean(pg/ml): 326, Standard deviation: 24.45, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 624, Standard deviation: 48.67, CV(%): 7.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour TRAIL Kit ELISA (ABIN921077)

Duan, Gu, Zhu, Sun, Chen, Feng, Song, Xu, He, He: "Schistosoma japonicum soluble egg antigens induce apoptosis and inhibit activation of hepatic stellate cells: a possible molecular mechanism." dans: International journal for parasitology, Vol. 44, Issue 3-4, pp. 217-24, (2014) (PubMed).

Sun, Zhang, Lv, Liu, Jin, Zhang, He, Deng, Liu, Li, Li, Zhou, Xie, Xie, Hu, Duan: "Toll-like receptor 3 (TLR3) induces apoptosis via death receptors and mitochondria by up-regulating the transactivating p63 isoform alpha (TAP63alpha)." dans: The Journal of biological chemistry, Vol. 286, Issue 18, pp. 15918-28, (2011) (PubMed).

Détail du antigène

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Autre désignation: TNFSF10 (TNFSF10 Produits)

Synonymes: APO2L Kit ELISA, Apo-2L Kit ELISA, CD253 Kit ELISA, TL2 Kit ELISA, TRAIL Kit ELISA, A330042I21Rik Kit ELISA, AI448571 Kit ELISA, APO-2L Kit ELISA, Ly81 Kit ELISA, Trail Kit ELISA, tl2 Kit ELISA, apo2l Kit ELISA, cd253 Kit ELISA, trail Kit ELISA, apo-2l Kit ELISA, trail1 Kit ELISA, xtrail1 Kit ELISA, tnfsf10l2 Kit ELISA, zgc:92320 Kit ELISA, TNLG6A Kit ELISA, TNF superfamily member 10 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 10 Kit ELISA, tumor necrosis factor superfamily member 10 Kit ELISA, TNF superfamily member 10 S homeolog Kit ELISA, TNFSF10 Kit ELISA, Tnfsf10 Kit ELISA, tnfsf10.S Kit ELISA, tnfsf10 Kit ELISA

Sujet:

Protein Function: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

Background: In the field of cell biology, TNF-related apoptosis-inducing ligand(TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253(cluster of differentiation 253).Tumor necrosis factor(TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4(TRAIL-RI) and DR5(TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain(DcR1) or contain a truncated death domain(DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28.

Synonyms: Tumor necrosis factor ligand superfamily member 10,Apo-2 ligand,Apo-2L,TNF-related apoptosis-inducing ligand,Protein TRAIL,CD253,TNFSF10,APO2L, TRAIL,

Full Gene Name: Tumor necrosis factor ligand superfamily member 10

Cellular Localisation: Membrane, Single-pass type II membrane protein.

ID gène: 8743

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity