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IL1R1 Kit ELISA
IL1R1
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730127
Détail du produit IL1R1 Kit ELISA
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Antigène
Voir toutes IL1R1 Kits ELISA
IL1R1
(Interleukin 1 Receptor, Type I (IL1R1))
Reactivité
Toutes les réactivités sur IL1R1 Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse IL-1 RI ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse IL-1 RI.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes IL1R1 Kits ELISA
IL1R1
(Interleukin 1 Receptor, Type I (IL1R1))
Autre désignation
IL-1 RI (IL1R1 Produits )
Synonymes
CD121A Kit ELISA, D2S1473 Kit ELISA, IL-1R-alpha Kit ELISA, IL1R Kit ELISA, IL1RA Kit ELISA, P80 Kit ELISA, CD121a Kit ELISA, CD121b Kit ELISA, IL-iR Kit ELISA, Il1r-1 Kit ELISA, DER4 Kit ELISA, Fit-1 Kit ELISA, Ly84 Kit ELISA, ST2L Kit ELISA, St2 Kit ELISA, St2-rs1 Kit ELISA, T1 Kit ELISA, T1/ST2 Kit ELISA, interleukin 1 receptor type 1 Kit ELISA, interleukin 1 receptor, type I Kit ELISA, interleukin 1 receptor-like 1 Kit ELISA, IL1R1 Kit ELISA, Il1r1 Kit ELISA, Il1rl1 Kit ELISA
UniProt
P13504
Pathways
Signalisation NF-kappaB , Carbohydrate Homeostasis , Cancer Immune Checkpoints
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