S100A8 Kit ELISA
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- Antigène Voir toutes S100A8 Kits ELISA
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.65 ng/mL - 150 ng/mL
- Seuil minimal de détection
- 0.65 ng/mL
- Application
- ELISA
- Fonction
- Mouse S100A8/S100A9 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Mouse S100A8.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product S100A8 Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Préparation des réactifs
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Recommended Dilution for serum and plasma samples2 fold
- Restrictions
- For Research Use only
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- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes S100A8 Kits ELISA
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
- Autre désignation
- S100A8 (S100A9 (S100A8 Produits)
- Synonymes
- 60B8AG Kit ELISA, CAGA Kit ELISA, CFAG Kit ELISA, CGLA Kit ELISA, CP-10 Kit ELISA, L1Ag Kit ELISA, MA387 Kit ELISA, MIF Kit ELISA, MRP8 Kit ELISA, NIF Kit ELISA, P8 Kit ELISA, Mrp8 Kit ELISA, S100A8 Kit ELISA, 60B8Ag Kit ELISA, AI323541 Kit ELISA, B8Ag Kit ELISA, CFAg Kit ELISA, Caga Kit ELISA, p8 Kit ELISA, MRP-8 Kit ELISA, S100 calcium binding protein A8 Kit ELISA, S100 calcium binding protein A8 (calgranulin A) Kit ELISA, S100A8 Kit ELISA, S100a8 Kit ELISA
- Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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