PRKAR1A Kit ELISA

N° du produit ABIN6730540

Détail du produit PRKAR1A Kit ELISA

Antigène: PRKAR1A (Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human PRKAR1A ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human PRKAR1A.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PRKAR1A (Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))

Autre désignation: PRKAR1A (PRKAR1A Produits)

Synonymes: ACRDYS1 Kit ELISA, ADOHR Kit ELISA, CAR Kit ELISA, CNC Kit ELISA, CNC1 Kit ELISA, PKR1 Kit ELISA, PPNAD1 Kit ELISA, PRKAR1 Kit ELISA, TSE1 Kit ELISA, 1300018C22Rik Kit ELISA, RIalpha Kit ELISA, Tse-1 Kit ELISA, Tse1 Kit ELISA, RIIA Kit ELISA, prkar1a Kit ELISA, zgc:92515 Kit ELISA, im:7047729 Kit ELISA, cnc1 Kit ELISA, pkr1 Kit ELISA, ppnad1 Kit ELISA, prkar1 Kit ELISA, tse1 Kit ELISA, protein kinase cAMP-dependent type I regulatory subunit alpha Kit ELISA, protein kinase, cAMP dependent regulatory, type I, alpha Kit ELISA, protein kinase cAMP-dependent type 1 regulatory subunit alpha Kit ELISA, protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a Kit ELISA, cAMP-dependent protein kinase type I-alpha regulatory subunit pseudogene Kit ELISA, protein kinase cAMP-dependent type I regulatory subunit alpha S homeolog Kit ELISA, PRKAR1A Kit ELISA, Prkar1a Kit ELISA, prkar1aa Kit ELISA, LOC707275 Kit ELISA, prkar1a.S Kit ELISA

UniProt: P10644

Pathways: Signalisation Hedgehog, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma