KHDRBS1 Kit ELISA

N° du produit ABIN6951166

Détail du produit KHDRBS1 Kit ELISA

Antigène: KHDRBS1 (KH Domain Containing, RNA Binding, Signal Transduction Associated 1 (KHDRBS1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human P62 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: KHDRBS1 (KH Domain Containing, RNA Binding, Signal Transduction Associated 1 (KHDRBS1))

Autre désignation: P62 (KHDRBS1 Produits)

Synonymes: Sam68 Kit ELISA, p62 Kit ELISA, p68 Kit ELISA, fj90g10 Kit ELISA, khdrbs1 Kit ELISA, wu:fa18g12 Kit ELISA, wu:fa56c01 Kit ELISA, wu:fc91b01 Kit ELISA, wu:fj90g10 Kit ELISA, zgc:113899 Kit ELISA, KHDRBS1 Kit ELISA, P62 Kit ELISA, khdrbs1l Kit ELISA, sb:cb97 Kit ELISA, wu:fb07d11 Kit ELISA, wu:fi43c05 Kit ELISA, zgc:85948 Kit ELISA, KH RNA binding domain containing, signal transduction associated 1 Kit ELISA, KH domain containing, RNA binding, signal transduction associated 1a Kit ELISA, KH domain containing, RNA binding, signal transduction associated 1 Kit ELISA, KH domain containing, RNA binding, signal transduction associated 1 S homeolog Kit ELISA, KH domain containing, RNA binding, signal transduction associated 1b Kit ELISA, KHDRBS1 Kit ELISA, khdrbs1a Kit ELISA, khdrbs1 Kit ELISA, Khdrbs1 Kit ELISA, khdrbs1.S Kit ELISA, khdrbs1b Kit ELISA

ID gène: 8878

UniProt: Q13501

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Autophagy