PKC alpha Kit ELISA

N° du produit ABIN6951211

Détail du produit PKC alpha Kit ELISA

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human PKC alpha ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Autre désignation: PKC alpha (PKCa Produits)

Synonymes: pkc-alpha Kit ELISA, pkcalpha Kit ELISA, im:7139045 Kit ELISA, si:dkey-8l19.1 Kit ELISA, LOC100219195 Kit ELISA, AAG6 Kit ELISA, PKC-alpha Kit ELISA, PKCA Kit ELISA, PRKACA Kit ELISA, AI875142 Kit ELISA, Pkca Kit ELISA, PKRCA Kit ELISA, protein kinase C alpha Kit ELISA, protein kinase C, alpha L homeolog Kit ELISA, protein kinase C, alpha Kit ELISA, PRKCA Kit ELISA, prkca.L Kit ELISA, prkca Kit ELISA, Prkca Kit ELISA

ID gène: 5578

UniProt: P17252

Pathways: Signalisation WNT, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling