RHEB Kit ELISA

N° du produit ABIN6951226

Détail du produit RHEB Kit ELISA

Antigène: RHEB (Ras Homolog Enriched in Brain (RHEB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human RHEB ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: RHEB (Ras Homolog Enriched in Brain (RHEB))

Autre désignation: RHEB (RHEB Produits)

Synonymes: RHEB2 Kit ELISA, RHEB Kit ELISA, rheb2 Kit ELISA, CG1081 Kit ELISA, DReb Kit ELISA, Dm Rheb Kit ELISA, DmRheb Kit ELISA, Dmel\CG1081 Kit ELISA, Q9VND8 Kit ELISA, RheB Kit ELISA, dRheb Kit ELISA, rheb Kit ELISA, Ras homolog, mTORC1 binding Kit ELISA, Ras homolog enriched in brain Kit ELISA, ras homolog enriched in brain Kit ELISA, Ras homolog, mTORC1 binding L homeolog Kit ELISA, RHEB Kit ELISA, Rheb Kit ELISA, rheb Kit ELISA, rheb.L Kit ELISA

ID gène: 6009

UniProt: Q15382

Pathways: Signalisation RTK