HRAS Kit ELISA

N° du produit ABIN6951446

Détail du produit HRAS Kit ELISA

Antigène: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human HRAS ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: HRAS (HRas proto-oncogene, GTPase (HRAS))

Autre désignation: HRAS (HRAS Produits)

Synonymes: C-BAS/HAS Kit ELISA, C-H-RAS Kit ELISA, C-HA-RAS1 Kit ELISA, CTLO Kit ELISA, H-RASIDX Kit ELISA, HAMSV Kit ELISA, HRAS1 Kit ELISA, K-RAS Kit ELISA, N-RAS Kit ELISA, RASH1 Kit ELISA, hras Kit ELISA, zgc:110250 Kit ELISA, HRAS Kit ELISA, H-RAS Kit ELISA, c-H-ras Kit ELISA, H-Ras Kit ELISA, K-Ras Kit ELISA, hras1 Kit ELISA, rash1 Kit ELISA, ras Kit ELISA, N-Ras Kit ELISA, c-bas/has Kit ELISA, H-ras Kit ELISA, Ha-ras Kit ELISA, Harvey-ras Kit ELISA, Hras-1 Kit ELISA, Kras2 Kit ELISA, c-Ha-ras Kit ELISA, c-rasHa Kit ELISA, hrasl Kit ELISA, zgc:110734 Kit ELISA, HRas proto-oncogene, GTPase Kit ELISA, v-Ha-ras Harvey rat sarcoma viral oncogene homolog a Kit ELISA, neuroblastoma RAS viral (v-ras) oncogene homolog pseudogene Kit ELISA, Harvey rat sarcoma viral oncogene homolog L homeolog Kit ELISA, Harvey rat sarcoma viral oncogene homolog Kit ELISA, Harvey rat sarcoma virus oncogene Kit ELISA, NRAS proto-oncogene, GTPase Kit ELISA, -Ha-ras Harvey rat sarcoma viral oncogene homolog b Kit ELISA, HRAS Kit ELISA, hrasa Kit ELISA, LOC733587 Kit ELISA, Hras Kit ELISA, hras.L Kit ELISA, hras Kit ELISA, NRAS Kit ELISA, hrasb Kit ELISA

ID gène: 3265

UniProt: P01112

Pathways: Signalisation p53, Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling