CDC42 Kit ELISA

N° du produit ABIN6951508

Détail du produit CDC42 Kit ELISA

Antigène: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human CDC42 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Autre désignation: CDC42 (CDC42 Produits)

Synonymes: CDC42Hs Kit ELISA, G25K Kit ELISA, AI747189 Kit ELISA, AU018915 Kit ELISA, cdc42a Kit ELISA, wu:fb07d08 Kit ELISA, wu:fj62g06 Kit ELISA, zgc:63918 Kit ELISA, Xcdc42 Kit ELISA, cdc42hs Kit ELISA, g25k Kit ELISA, CDC42 Kit ELISA, CG12530 Kit ELISA, Cdc42Dm Kit ELISA, D-CDC42 Kit ELISA, D-Cdc42 Kit ELISA, D-cdc42 Kit ELISA, DCDC42 Kit ELISA, DCdc42 Kit ELISA, Dcdc42 Kit ELISA, Dm Cdc42 Kit ELISA, DmCDC42 Kit ELISA, Dmcdc42 Kit ELISA, Dmel\\CG12530 Kit ELISA, cdc42 Kit ELISA, cell division cycle 42 Kit ELISA, Cell division control protein 42 homolog Kit ELISA, cell division control protein 42 homolog Kit ELISA, cell division cycle 42 L homeolog Kit ELISA, CG12530 gene product from transcript CG12530-RC Kit ELISA, Rho family GTPase Kit ELISA, CDC42 Kit ELISA, Cdc42 Kit ELISA, cdc42 Kit ELISA, LOC100636735 Kit ELISA, LOC100637699 Kit ELISA, LOC100638834 Kit ELISA, cdc42.L Kit ELISA

ID gène: 998

UniProt: P60953

Pathways: Signalisation MAPK, Dynamique des Microtubules, Signalisation RTK, Signalisation WNT, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling