PCSK9 [Biotinylated] : LDL R Inhibitor Screening ELISA Assay Pair

N° du produit ABIN7035518

Détail du produit

Antigène: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Reactivité: Humain

Attributs du produit: This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of new PCSK9 pathway inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized human LDL R and in-house developed biotinylated PCSK9 protein. This product is uniquely suitable for rapid high-throughput screening of putative PCSK9 inhibitors. Briefly, we provide you with a biotinylated human PCSK9 protein, a human LDL R protein, an anti-PCSK9 neutralizing antibody (as method verified Reference), and Streptavidin-HRP reagent. Your experiment will include 4 simple steps:
a) Coat the plate with human LDL R.
b) Add your molecule of interest to the plates.
c) Add human PCSK9-Biotin to the plates.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit PCSK9: LDL R binding will be determined by comparing OD readings among different experimental groups.

Ingrédients:

• Human LDL R
• Biotinylated Human PCSK9
• Streptavidin-HRP
• Anti-PCSK9 Neutralizing Antibody

Information d'application

Indications d'application: This pair is useful for screening for inhibitors of human PCSK9 binding to human LDL R.

Restrictions: For Research Use only

Stockage

Stock: -80 °C

Stockage commentaire: No activity loss was observed after storage at: -20°C for 1 year in lyophilized state after receipt, -70°C for 1 year under sterile conditions after reconstitution with carrier protein., Powder,RT

Détail du antigène

Antigène: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Autre désignation: PCSK9 (PCSK9 Produits)

Synonymes: FH3 Kit, HCHOLA3 Kit, LDLCQ1 Kit, NARC-1 Kit, NARC1 Kit, PC9 Kit, AI415265 Kit, AI747682 Kit, Narc1 Kit, proprotein convertase subtilisin/kexin type 9 Kit, proprotein convertase subtilisin/kexin type 9 L homeolog Kit, PCSK9 Kit, pcsk9.L Kit, pcsk9 Kit, Pcsk9 Kit

Sujet: PCSK9 is a crucial protein in the regulation of plasma cholesterol homeostasis. It binds low density lipoprotein receptor (LDL R) to enhance the degradation of LDL R. Therefore, inhibition of PCSK9 has been considered a promising strategy to prevent the receptor from being degraded and promote removal of LDL cholesterol from circulation.