BAD Kit ELISA

N° du produit ABIN7830435

Le kit ELISA BAD (ABIN7830435) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain BAD.

Aperçu rapide pour BAD Kit ELISA (ABIN7830435)

Antigène: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit BAD Kit ELISA

Fonction: BAD (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BAD expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Autre désignation: BAD

Sujet:

Synonyms: BBC2|BCL2L8

Gene Description: BCL2-associated agonist of cell death

ID gène: 572

UniProt: Q92934

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, Apoptose, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events