EGFR Kit ELISA

N° du produit ABIN7830618

Le kit ELISA EGFR (ABIN7830618) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain EGFR.

Aperçu rapide pour EGFR Kit ELISA (ABIN7830618)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit EGFR Kit ELISA

Fonction: EGFR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EGFR expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR

Sujet:

Synonyms: ERBB|ERBB1|HER1|PIG61|mENA

Gene Description: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins