TRAF6 Kit ELISA
Aperçu rapide pour TRAF6 Kit ELISA (ABIN8088047)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 0.156 ng/mL
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Fonction
- Human TNF Receptor Associated Factor 6 (TRAF6) ELISA Kit for detecting TRAF6 in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensibilité
- 0.054 ng/mL
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Ingrédients
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Matériel non inclus
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Commentaires
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 h
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Plaque
- Pre-coated
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Précision du teste
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- 4°C for one month, Long term: see manual.
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Date de péremption
- 12 months
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- TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))
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Autre désignation
- TNF Receptor Associated Factor 6
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Sujet
- Gene Aliases: RNF85, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer
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ID gène
- 7189
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UniProt
- Q9Y4K3
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Pathways
- Signalisation NF-kappaB, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway
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