Cytochrome P450 Kit ELISA

N° du produit ABIN2630930

Détail du produit Cytochrome P450 Kit ELISA

Antigène: Cytochrome P450 (CYP)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Fonction: For the quantitative determination of human cytochrome P450 concentrations in serum, plasma, tissue homogenates, cell lysates.

Type d'échantillon: Serum, Plasma, Tissue Homogenate, Cell Lysate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of human Cytochrome P450.

Réactivité croisée (Details): Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.

Sensibilité: 7.8 pg/mL

Ingrédients:

• Assay plate (12 × 8 coated Microwells)
• Standard (freeze dried)
• Biotin-antibody (100 × concentrate)
• HRP-avidin (100 × concentrate)
• Biotin-antibody Diluent
• HRP-avidin Diluent
• Sample Diluent
• Wash Buffer (25 × concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip (for 96 wells)
• Instruction manual

Information d'application

Indications d'application:

• The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
• Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
• Grossly hemolyzed samples are not suitable for use in this assay.
• If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
• Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
• Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
• Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
• Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
• Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

Commentaires:

Detection wavelength: 450 nm

Information on standard material:
Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

Information on reagents:
In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

Information on antibodies:
The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Cytochrome P450 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cytochrome P450 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Cytochrome P450 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cytochrome P450 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Précision du teste: Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.

• Intra-assay: CV% less than 8%
• Inter-assay: CV% less than 10%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Conseil sur la manipulation:

• The kit should not be used beyond the expiration date on the kit label.
• Do not mix or substitute reagents with those from other lots or sources.
• If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
• Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
• This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

Stock: 4 °C/-20 °C

Stockage commentaire: For unopened kit: All the reagents should be kept according to the labels on vials.

Date de péremption: 6 months

Détail du antigène

Antigène: Cytochrome P450 (CYP)

Autre désignation: Cytochrome P450 (CYP Produits)

Synonymes: CPF3 Kit ELISA, CYP4F Kit ELISA, LTB4H Kit ELISA, 6t1 Kit ELISA, CG1644 Kit ELISA, DmelCG1644 Kit ELISA, CYTOCHROME P450 Kit ELISA, FAMILY 705 Kit ELISA, POLYPEPTIDE 9 Kit ELISA, SUBFAMILY A Kit ELISA, T20P8.6 Kit ELISA, T20P8_6 Kit ELISA, cytochrome P450 Kit ELISA, family 71 Kit ELISA, polypeptide 13 Kit ELISA, subfamily A Kit ELISA, T11J7.16 Kit ELISA, T11J7_16 Kit ELISA, F13P17.22 Kit ELISA, family 710 Kit ELISA, polypeptide 2 Kit ELISA, CYP77A7 Kit ELISA, FAMILY 77 Kit ELISA, POLYPEPTIDE 7 Kit ELISA, UNE9 Kit ELISA, UNFERTILIZED EMBRYO SAC 9 Kit ELISA, POLYPEPTIDE 15 Kit ELISA, POLYPEPTIDE 18 Kit ELISA, T9E8.50 Kit ELISA, T9E8_50 Kit ELISA, polypeptide 20 Kit ELISA, DL3710C Kit ELISA, FCAALL.270 Kit ELISA, POLYPEPTIDE 1 Kit ELISA, CABBAGE 3 Kit ELISA, CBB3 Kit ELISA, CONSTITUTIVE PHOTOMORPHOGENIC DWARF Kit ELISA, CYP90 Kit ELISA, CYP90A Kit ELISA, CYP90A1 Kit ELISA, CYTOCHROME P450 90A1 Kit ELISA, CYTOCHROME P450 MONOOXYGENASE Kit ELISA, DWARF 3 Kit ELISA, DWF3 Kit ELISA, MJJ3.9 Kit ELISA, MJJ3_9 Kit ELISA, F25C20.24 Kit ELISA, F25C20_24 Kit ELISA, polypeptide 18 Kit ELISA, F22L4.14 Kit ELISA, F22L4_14 Kit ELISA, FAMILY 86 Kit ELISA, POLYPEPTIDE 4 Kit ELISA, F13D4.120 Kit ELISA, F13D4_120 Kit ELISA, family 82 Kit ELISA, polypeptide 1 Kit ELISA, subfamily F Kit ELISA, family 711 Kit ELISA, MAX1 Kit ELISA, MORE AXILLARY BRANCHES 1 Kit ELISA, T1D16.19 Kit ELISA, T1D16_19 Kit ELISA, F15K20.21 Kit ELISA, F15K20_21 Kit ELISA, family 94 Kit ELISA, subfamily C Kit ELISA, F13P17.35 Kit ELISA, family 98 Kit ELISA, polypeptide 3 Kit ELISA, T20B5.9 Kit ELISA, T20B5_9 Kit ELISA, family 712 Kit ELISA, T24P15.16 Kit ELISA, T24P15_16 Kit ELISA, family 704 Kit ELISA, T13E15.10 Kit ELISA, F17K2.4 Kit ELISA, F4I18.5 Kit ELISA, family 86 Kit ELISA, LACERATA Kit ELISA, LCR Kit ELISA, polypeptide 8 Kit ELISA, F14M4.22 Kit ELISA, family 709 Kit ELISA, subfamily B Kit ELISA, F14M4.21 Kit ELISA, family 87 Kit ELISA, polypeptide 9 Kit ELISA, T21P5.11 Kit ELISA, T21P5_11 Kit ELISA, F1C9.32 Kit ELISA, F1C9_32 Kit ELISA, POLYPEPTIDE 22 Kit ELISA, family 705 Kit ELISA, polypeptide 23 Kit ELISA, polypeptide 30 Kit ELISA, subfamily G Kit ELISA, family 76 Kit ELISA, subfamily D Kit ELISA, family 706 Kit ELISA, polypeptide 4 Kit ELISA, T4C9.140 Kit ELISA, T4C9_140 Kit ELISA, F18A5.160 Kit ELISA, F18A5_160 Kit ELISA, family 83 Kit ELISA, REDUCED EPIDERMAL FLUORESCENCE 2 Kit ELISA, REF2 Kit ELISA, DL3695C Kit ELISA, family 702 Kit ELISA, FCAALL.257 Kit ELISA, DL3700W Kit ELISA, FCAALL.266 Kit ELISA, DL3720W Kit ELISA, FCAALL.274 Kit ELISA, DL3725W Kit ELISA, FCAALL.277 Kit ELISA, 21-OH Kit ELISA, 21OH Kit ELISA, 21OHA Kit ELISA, 21OHB Kit ELISA, CYP21OH-A Kit ELISA, Cyp21 Kit ELISA, Cyp21-ps1 Kit ELISA, Cyp21B Kit ELISA, Cyp21a2-ps Kit ELISA, Cyp21a2ps Kit ELISA, Oh21-1 Kit ELISA, Oh21-2 Kit ELISA, CYPIID25 Kit ELISA, Cypiiia15 Kit ELISA, cytochrome P450 family 4 subfamily F member 3 Kit ELISA, CG1644 gene product from transcript CG1644-RA Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 9 Kit ELISA, cytochrome P450 family 71 polypeptide Kit ELISA, cytochrome P450, family 710, subfamily A, polypeptide 2 Kit ELISA, Cytochrome P450 superfamily protein Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 15 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 18 Kit ELISA, cytochrome P450, family 71, subfamily A, polypeptide 20 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 71, subfamily A, polypeptide 18 Kit ELISA, cytochrome P450, family 86, subfamily A, polypeptide 4 Kit ELISA, cytochrome P450, family 82, subfamily F, polypeptide 1 Kit ELISA, cytochrome P450, family 711, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 94, subfamily C, polypeptide 1 Kit ELISA, cytochrome P450, family 710, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 98, subfamily A, polypeptide 3 Kit ELISA, cytochrome P450, family 712, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 704, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 704, subfamily A, polypeptide 2 Kit ELISA, cytochrome P450, family 86, subfamily A, polypeptide 8 Kit ELISA, cytochrome P450, family 709, subfamily B, polypeptide 2 Kit ELISA, cytochrome P450, family 709, subfamily B, polypeptide 1 Kit ELISA, cytochrome P450, family 87, subfamily A, polypeptide 9 Kit ELISA, cytochrome P450, family 94, subfamily B, polypeptide 2 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 22 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 23 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 30 Kit ELISA, cytochrome P450, family 82, subfamily G, polypeptide 1 Kit ELISA, cytochrome P450, family 86, subfamily C, polypeptide 2 Kit ELISA, cytochrome P450, family 94, subfamily B, polypeptide 3 Kit ELISA, cytochrome P450, family 76, subfamily G, polypeptide 1 Kit ELISA, cytochrome P450, family 94, subfamily D, polypeptide 2 Kit ELISA, cytochrome P450, family 706, subfamily A, polypeptide 4 Kit ELISA, cytochrome P450, family 83, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 702, subfamily A, polypeptide 2 Kit ELISA, cytochrome P450, family 702, subfamily A, polypeptide 3 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 2 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 3 Kit ELISA, cytochrome P450, family 21, subfamily a, polypeptide 1 Kit ELISA, cytochrome P450, family 2, subfamily g, polypeptide 1 Kit ELISA, vitamin D3 25-Hydroxylase Kit ELISA, cytochrome P-450 Kit ELISA, cytochrome P450 Kit ELISA, olfactory receptor 1440-like Kit ELISA, CYP4F3 Kit ELISA, Cyp6t1 Kit ELISA, CYP705A9 Kit ELISA, CYP71A13 Kit ELISA, CYP710A2 Kit ELISA, UNE9 Kit ELISA, CYP705A15 Kit ELISA, CYP705A18 Kit ELISA, CYP71A20 Kit ELISA, CYP705A1 Kit ELISA, CPD Kit ELISA, CYP71A18 Kit ELISA, CYP86A4 Kit ELISA, CYP82F1 Kit ELISA, CYP711A1 Kit ELISA, CYP94C1 Kit ELISA, CYP710A1 Kit ELISA, CYP98A3 Kit ELISA, CYP712A1 Kit ELISA, CYP704A1 Kit ELISA, CYP704A2 Kit ELISA, CYP86A8 Kit ELISA, CYP709B2 Kit ELISA, CYP709B1 Kit ELISA, CYP89A9 Kit ELISA, CYP94B2 Kit ELISA, CYP705A22 Kit ELISA, CYP705A23 Kit ELISA, CYP705A30 Kit ELISA, CYP82G1 Kit ELISA, CYP86C2 Kit ELISA, CYP94B3 Kit ELISA, CYP76G1 Kit ELISA, CYP94D2 Kit ELISA, CYP706A4 Kit ELISA, CYP83A1 Kit ELISA, CYP702A2 Kit ELISA, CYP702A3 Kit ELISA, CYP705A2 Kit ELISA, CYP705A3 Kit ELISA, Cyp21a1 Kit ELISA, Cyp2g1 Kit ELISA, CYP2D25 Kit ELISA, LOC100328948 Kit ELISA, CYP1A1 Kit ELISA, Cyp3a15 Kit ELISA, LOC100068520 Kit ELISA

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process