p53 Kit ELISA

N° du produit ABIN921118

Kit ELISA p53 Humain, Colorimetric test pour la quantification de Humain p53 et a été mentionné dans 7+ publications.

Aperçu rapide pour p53 Kit ELISA (ABIN921118)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Épitope: AA 1-393

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate

Détail du produit p53 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich Human P53 ELISA Kit to quantitate Human TP53 in cell lysates.

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: M1-D393

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 6%, 4.7%, 7.8%
Inter-Assay Precision (CV%): 6.9%, 8.6%, 8.6%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour p53 Kit ELISA (ABIN921118)

Hasanzadeh, Baghban, Mokhtarzadeh, Shadjou, Mahboob: "An innovative immunosensor for detection of tumor suppressor protein p53 in unprocessed human plasma and cancer cell lysates." dans: International journal of biological macromolecules, Vol. 105, Issue Pt 1, pp. 1337-1348, (2018) (PubMed).

Lu, Lin, Gu, Li, Guo, Wang, Qiu: "Antitumor effects of crocin on human breast cancer cells." dans: International journal of clinical and experimental medicine, Vol. 8, Issue 11, pp. 20316-22, (2016) (PubMed).

Srivastava, Narang, Bhatia, Saluja: "Expression of Heat Shock Protein 70 Gene and Its Correlation with Inflammatory Markers in Essential Hypertension." dans: PLoS ONE, Vol. 11, Issue 3, pp. e0151060, (2016) (PubMed).

Sun, Yang, Wang, Sun, Dong: "Crocin attenuates cisplatin-induced liver injury in the mice." dans: Human & experimental toxicology, Vol. 33, Issue 8, pp. 855-62, (2015) (PubMed).

Fu, Chen, Cheng, Wang: "Hypoxia-inducible factor-1? protects cervical carcinoma cells from apoptosis induced by radiation via modulation of vascular endothelial growth factor and p53 under hypoxia." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 318-25, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." dans: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Li, Chu, Gao, Li: "Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin." dans: Acta biochimica et biophysica Sinica, Vol. 42, Issue 1, pp. 80-9, (2009) (PubMed).

Détail du antigène

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: TP53

Sujet:

Background: P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton ( kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.

Gene Full Name: tumor protein p53

ID gène: 7157

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg