Insulin Kit ELISA

N° du produit ABIN997069

Détail du produit Insulin Kit ELISA

Antigène: Insulin (INS)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0-200 μIU/mL

Seuil minimal de détection: 0 μIU/mL

Application: ELISA

Fonction: Insulin Microplate Elisa test is intended to be used for the quantitative determination of insulin levels in human serum.

Type d'échantillon: Serum

Analytical Method: Quantitative

Sensibilité: 1.5 μIU/mL

Information d'application

Volume d'échantillon: 25 μL

Durée du test: < 1 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12-14 months

Détail du antigène

Antigène: Insulin (INS)

Autre désignation: Insulin (INS Produits)

Synonymes: IDDM2 Kit ELISA, ILPR Kit ELISA, IRDN Kit ELISA, MODY10 Kit ELISA, ins1 Kit ELISA, xins Kit ELISA, ins1-a Kit ELISA, Insulin Kit ELISA, AA986540 Kit ELISA, Ins-2 Kit ELISA, InsII Kit ELISA, Mody Kit ELISA, Mody4 Kit ELISA, proinsulin Kit ELISA, zgc:109842 Kit ELISA, igf2-A Kit ELISA, ins Kit ELISA, ins-a Kit ELISA, ins-b Kit ELISA, insulin Kit ELISA, insulin precursor Kit ELISA, insulin II Kit ELISA, preproinsulin Kit ELISA, insulin L homeolog Kit ELISA, insulin S homeolog Kit ELISA, INS Kit ELISA, INS-IGF2 Kit ELISA, ins Kit ELISA, Ins Kit ELISA, PIN Kit ELISA, Ins2 Kit ELISA, ins.L Kit ELISA, ins.S Kit ELISA

Sujet: Insulin is the principal hormone responsible for glucose metabolism. It is synthesized in the cells of the islets of Langerhans as the precuror, proinsulin, which is processed to form C-peptide and insulin and both are secreted in equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chairs, the A chain (21 amino acids) and the B chain (30 amino acids), which are linked by two inter-chain disulphide bridges. There is, in addition, a single intra-chain disulphide bridge in the A chain. The sequence of insulin is highly conserved in mammalian species, and is homologous with the insulin-like growth factors IGF-I and IGF-II. Secretion of insulin is mainly controlled by plasma glucose concentration and the hormones have a number of important metabolic actions. Its principal function is to control the uptake and utilization of glucose in peripheral tissues via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagons, epinephrine(adrenaline), growth hormone and cortisol. Insulin concentrations are severely reduced in insulin-dependent diabetes (DDM) and some other conditions such as hypopituitarism. Insulin concentrations may be raised in non-insulin- dependant diabetes (NIDDM), obesity, insulinoma and some endocrine dysfunctions such as Cushing?s Syndrome and Acromegaly.

The main clinical utility measurement is in the investigation of hypoglycaemia. Insulin assay have been used in the following applications:
1. To assess the residual cell function, especially in newly diagnosed cases of IDDM.
2. As an aid to the discrimination between IDDM and NIDDM.
3. The diagnosis of insulinoma.
4. In the investigation of the pathophysiology of diabetes mellitus.
Insulin assays are the essentials in various dynamic tests, such as oral of intravenous glucose tolerance tests (OGTT and IVGTT), to determine the insulin response of the pancreas and the degree of insulin resistance. In many applications, insulin measurements may be complicated by cross-reactivity with partially degraded insulin, proinsulin and split forms of proinsulin. Immune complexes of these molecules are essentially problematic in patients who have developed anti-insulin antibodies through animal insulin administration.

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation