When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
TRPM8
(Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8))
Synonymes
TRPM8 Peptide, trpp8 Peptide, ltrpc6 Peptide, trpm8b Peptide, LTRPC6 Peptide, TRPP8 Peptide, CMR1 Peptide, LTrpC-6 Peptide, Trp-p8 Peptide, transient receptor potential cation channel subfamily M member 8 Peptide, transient receptor potential cation channel subfamily M member 8b L homeolog Peptide, transient receptor potential cation channel, subfamily M, member 8 Peptide, TRPM8 Peptide, trpm8b.L Peptide, Trpm8 Peptide