TFPI Protein (AA 29-280, partial) (GST tag)

N° du produit ABIN1047743

Détail du produit

Antigène: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Type de proteíne: Recombinant

Attributs du protein: AA 29-280, partial

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette TFPI protéine est marqué à la GST tag.

Application: ELISA

Séquence: DSEEDEEHTI ITDTELPPLK LMHSFCAFKA DDGPCKAIMK RFFFNIFTRQ CEEFIYGGCE GNQNRFESLE ECKKMCTRDN ANRIIKTTLQ QEKPDFCFLE EDPGICRGYI TRYFYNNQTK QCERFKYGGC LGNMNNFETL EECKNICEDG PNGFQVDNYG TQLNAVNNSL TPQSTKVPSL FEFHGPSWCL TPADRGLCRA NENRFYYNSV IGKCRPFKYS GCGGNENNFT SKQECLRACK KGFIQRISKG GL

Attributs du produit: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Pureté: 90 %

Information d'application

Commentaires:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Conseil sur la manipulation: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Stock: -20 °C

Stockage commentaire: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Référence

van der Logt, Reitsma, Bertina: "Intron-exon organization of the human gene coding for the lipoprotein-associated coagulation inhibitor: the factor Xa dependent inhibitor of the extrinsic pathway of coagulation." dans: Biochemistry, Vol. 30, Issue 6, pp. 1571-7, (1991) (PubMed).

Girard, Warren, Novotny, Bejcek, Miletich, Broze: "Identification of the 1.4 kb and 4.0 kb messages for the lipoprotein associated coagulation inhibitor and expression of the encoded protein." dans: Thrombosis research, Vol. 55, Issue 1, pp. 37-50, (1989) (PubMed).

Wun, Kretzmer, Girard, Miletich, Broze: "Cloning and characterization of a cDNA coding for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains." dans: The Journal of biological chemistry, Vol. 263, Issue 13, pp. 6001-4, (1988) (PubMed).

Détail du antigène

Antigène: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Autre désignation: Tissue factor pathway inhibitor protein (TFPI Produits)

Synonymes: EPI Protein, LACI Protein, TFI Protein, TFPI1 Protein, A630013F22Rik Protein, AW552122 Protein, TFPI Protein, si:dz44f8.1 Protein, tfpib Protein, wu:fb92g01 Protein, zgc:152844 Protein, zgc:158592 Protein, tissue factor pathway inhibitor Protein, tissue factor pathway inhibitor a Protein, TFPI Protein, Tfpi Protein, tfpia Protein

Sujet: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

Poids moléculaire: 56.3 kD

UniProt: P10646