TNF alpha Protein (AA 77-235)

N° du produit ABIN1097669

Cette protéine Recombinant TNF alpha est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour TNF alpha Protein (AA 77-235) (ABIN1097669)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Origine: Lapin

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 77-235

Fonction: Recombinant Rabbit Tumor Necrosis Factor α/TNF-α

Séquence: MVTLRSASRA LSDKPLAHVV ANPQVEGQLQ WLSQRANALL ANGMKLTDNQ LVVPADGLYL IYSQVLFSGQ GCRSYVLLTH TVSRFAVSYP NKVNLLSAIK SPCHRETPEE AEPMAWYEPI YLGGVFQLEK GDRLSTEVNQ PEYLDLAESG QVYFGIIAL

Attributs du produit:

Recombinant Rabbit Tumor Necrosis Factor α/TNF-α

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, pH 7.4.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor alpha/tnf-alpha

Sujet: Recombinant Rabbit Tumor Necrosis Factor α/TNF-α is a non-glycosylated cytokine produced from E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 AAs combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17.4 Daltons.
Tumor necrosis factor α (TNFα) is the prototypic ligand of the TNF superfamily. TNFα forms a homotrimer and functions by activating two types of receptors TNF-R1 (TNF receptor type 1,p55R) and TNF-R2 (TNF receptor type 2,p75R). TNFα is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.

Poids moléculaire: 17.59 kDa

UniProt: P04924

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome