HIF1A Protein (AA 1-735) (GST tag)

N° du produit ABIN1306618

Détail du produit

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Type de proteíne: Recombinant

Attributs du protein: AA 1-735

Origine: Humain

Source: Wheat germ

Purification/Conjugué: Cette HIF1A protéine est marqué à la GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Fonction: HIF1A (Human) Recombinant Protein (P01)

Séquence: MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMKAQMNC FYLKALDGFV MVLTDDGDMI YISDNVNKYM GLTQFELTGH SVFDFTHPCD HEEMREMLTH RNGLVKKGKE QNTQRSFFLR MKCTLTSRGR TMNIKSATWK VLHCTGHIHV YDTNSNQPQC GYKKPPMTCL VLICEPIPHP SNIEIPLDSK TFLSRHSLDM KFSYCDERIT ELMGYEPEEL LGRSIYEYYH ALDSDHLTKT HHDMFTKGQV TTGQYRMLAK RGGYVWVETQ ATVIYNTKNS QPQCIVCVNY VVSGIIQHDL IFSLQQTECV LKPVESSDMK MTQLFTKVES EDTSSLFDKL KKEPDALTLL APAAGDTIIS LDFGSNDTET DDQQLEEVPL YNDVMLPSPN EKLQNINLAM SPLPTAETPK PLRSSADPAL NQEVALKLEP NPESLELSFT MPQIQDQTPS PSDGSTRQSS PEPNSPSEYC FYVDSDMVNE FKLELVEKLF AEDTEAKNPF STQDTDLDLE MLAPYIPMDD DFQLRSFDQL SPLESSSASP ESASPQSTVT VFQQTQIQEP TANATTTTAT TDELKTVTKD RMEDIKILIA SPSPTHIHKE TTSATSSPYR DTQSRTASPN RAGKGVIEQT EKSHPRSPNV LSVALSQRTT VPEEELNPKI LALQNAQRKR KMEHDGSLFQ AVGII

Attributs du produit: Human HIF1A full-length ORF ( NP_851397.1, 1 a.a. - 735 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Stockage

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Conseil sur la manipulation: Aliquot to avoid repeated freezing and thawing.

Stock: -80 °C

Stockage commentaire: Best use within three months from the date of receipt of this protein.

Référence

Vadivel, Alphonse, Etches, van Haaften, Collins, OReilly, Eaton, Thébaud: "Hypoxia-inducible factors promote alveolar development and regeneration." dans: American journal of respiratory cell and molecular biology, Vol. 50, Issue 1, pp. 96-105, (2014) (PubMed).

Yang, Li, Yin, Huang, Sun, Li: "A new method to assay hypoxia-inducible factor-1 based on small molecule binding DNA." dans: Analytica chimica acta, Vol. 838, pp. 31-6, (2014) (PubMed).

Yang, Wang, Humphries, Hogan, OShea, Moynagh: "The E3 ubiquitin ligase Pellino3 protects against obesity-induced inflammation and insulin resistance." dans: Immunity, Vol. 41, Issue 6, pp. 973-87, (2014) (PubMed).

Détail du antigène

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Autre désignation: HIF1A (HIF1A Produits)

Synonymes: hif1a Protein, AA959795 Protein, HIF1alpha Protein, MOP1 Protein, bHLHe78 Protein, HIF-1A Protein, HIF-1alpha Protein, HIF1 Protein, HIF1-ALPHA Protein, PASD8 Protein, HIF1-alpha Protein, hif-1a Protein, hypoxia inducible factor 1 alpha Protein, hypoxia inducible factor 1, alpha subunit Protein, hypoxia inducible factor 1 alpha subunit Protein, HIF (Hypoxia Inducible Factor) homologa Protein, hif-1a Protein, Hif1a Protein, HIF1A Protein, hif1a Protein, hif-1 Protein

Sujet: Full Gene Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms: HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78

ID gène: 3091

NCBI Accession: NM_181054

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg