TNF alpha Protein (AA 89-235)

N° du produit ABIN1691953

Protéine Recombinant TNF alpha exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour TNF alpha Protein (AA 89-235) (ABIN1691953)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 89-235

Fonction: Recombinant Mouse Tumor Necrosis Factor/TNFα

Séquence: MDKPVAHVVA NHQVEEQLEW LSQRANALLA NGMDLKDNQL VVPADGLYLV YSQVLFKGQG CPDYVLLTHT VSRFAISYQE KVNLLSAVKS PCPKDTPEGA ELKPWYEPIY LGGVFQLEKG DQLSAEVNLP KYLDFAESGQ VYFGVIAL

Attributs du produit:

Recombinant Mouse Tumor Necrosis Factor/TNFα

The ED50 for this effect is typically 6.8 pg/mL.Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor/Tnf

Sujet: Recombinant Mouse Tumor Necrosis Factor/Tnf is produced with our E. coli expression system. The target protein is expressed with sequence (Asp89-Leu235) of Mouse Tnf.
Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

Poids moléculaire: 16.4 kDa

UniProt: P06804

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome