LacI (AA 1-360) protein (His tag)

N° du produit ABIN1692434

Détail du produit

Antigène: LacI

Type de proteíne: Recombinant

Attributs du protein: AA 1-360

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: His tag

Séquence: MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH

Attributs du produit: Recombinant E.coli Lactose operon repressor/LacI is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Gln360) of E.coli LacI fused with a 6His tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 5 mM DTT pH 8.0 .

Conseil sur la manipulation: Always centrifuge tubes before opening.
Do not mix by vortex or pipetting.
Please aliquot to minimize freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: LacI

Synonymes: ECK0342 Protein, JW0336 Protein, lactose-inducible lac operon transcriptional repressor Protein, lacI Protein

Sub Type: Fusionprotein

Sujet: Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
Alternative Names: Lactose operon repressor, LacI

Poids moléculaire: 39.4 kDa

UniProt: P03023