Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.
Pureté
> 95 % by SDS-PAGE and HPLC analyses.
Stérilité
0.2 μm filtered
niveau d'endotoxine
< 1 EU/μg of rHuFGF-18 as determined by LAL method.
FGF18
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>95 % as determined by reducing SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.
Fibroblast growth factor 18 (FGF18) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Synonyms: zFGF5, FGFI
Poids moléculaire
21.1 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.