HGF Protein (AA 32-494, AA 495-728)

N° du produit ABIN2017955

Détail du produit

Antigène: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 32-494, AA 495-728

Origine: Humain

Source: CHO Cells

Attributs du produit: ED50 < 10 ng/mL, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1x10^5 units/mg.
Alpha chain: AA 32-494, beta chain: AA 495-728

Pureté: > 95 % as analyzed by SDS-PAGE.

niveau d'endotoxine: < 0.2 EU/μg, determined by LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant humanHepatocyte Growth Factor (HGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, human Hepatocyte Growth Factor (HGF) should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Autre désignation: Hepatocyte Growth Factor (HGF) (HGF Produits)

Synonymes: DFNB39 Protein, F-TCF Protein, HGFB Protein, HPTA Protein, SF Protein, HGF Protein, C230052L06Rik Protein, HGF/SF Protein, NK1 Protein, NK2 Protein, SF/HGF Protein, hepatocyte growth factor Protein, HGF Protein, Hgf Protein

Sujet: Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.Recombinant human Hepatocyte Growth Factor (rhHGF) is produced in CHO cells and consists of two polypeptide chains (alpha-chain and beta-chain) held by a single disulfide bond resulting in the formation of a biologically active heterodimer. The alpha-chain consists of 463 amino acid residues and four kringle domains. The beta-chain consists of 234 amino acid residues. A fully biologically active molecule, rhHGF has a molecular mass of around 88-90 kDa analyzed by reducing SDS-PAGE.
Synonyms:

Poids moléculaire: 88-90 kDa (single chain), 59-61kDa (alpha chain), 30-34kDa (beta chain), observed by reducing SDS-PAGE.

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor