TNF alpha Protein (AA 84-235)

N° du produit ABIN2018408

Cette protéine Recombinant TNF alpha est produite dans Yeast (Pichia pastoris). Il y a 1 publication disponible.

Aperçu rapide pour TNF alpha Protein (AA 84-235) (ABIN2018408)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Yeast (Pichia pastoris)

Pureté: > 95 % as analyzed by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 84-235

Attributs du produit: ED50<0.01 ng/mL, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of >1 x 108 units/mg.

niveau d'endotoxine: < 1 EU/μg, determined by LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmTNF-alpha should be stable up to 1 week at 4°C or up to 2 months at -20°C.

Date de péremption: 6 months

Référence

Gao, Wan, Mateo, Callegari, Wang, Deng, Puente, Li, Chaussee, Finlay, Lenardo, Hardwidge: "Bacterial effector binding to ribosomal protein s3 subverts NF-kappaB function." dans: PLoS pathogens, Vol. 5, Issue 12, pp. e1000708, (2009) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF-alpha

Sujet: Sharing 79 % sequence identity with human Tumor Necrosis Factor-alpha (hTNF-α), mouse Tumor Necrosis Factor-alpha (mTNF-α) is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-α is further proteolytically processed to a soluble form [2]. The trimeric active TNF-α[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4]. Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Synonyms: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2

Poids moléculaire: 17kDa, observed by reducing SDS-PAGE.

UniProt: P06804

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome