Poliovirus Receptor Protein (PVR) (AA 21-343) (His tag)

N° du produit ABIN2180698

Détail du produit

Antigène: Poliovirus Receptor (PVR)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 21-343

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette Poliovirus Receptor protéine est marqué à la His tag.

Séquence: AA 21-343

Attributs du produit: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Référence

Chandramohan, Bryant, Piao, Keir, Lipp, Lefaivre, Perkinson, Bigner, Gromeier, McLendon: "Validation of an Immunohistochemistry Assay for Detection of CD155, the Poliovirus Receptor, in Malignant Gliomas." dans: Archives of pathology & laboratory medicine, Vol. 141, Issue 12, pp. 1697-1704, (2017) (PubMed).

Détail du antigène

Antigène: Poliovirus Receptor (PVR)

Autre désignation: CD155 (PVR Produits)

Synonymes: PVR Protein, CD155 Protein, HVED Protein, NECL5 Protein, Necl-5 Protein, PVS Protein, TAGE4 Protein, 3830421F03Rik Protein, D7Ertd458e Protein, Taa1 Protein, Tage4 Protein, mE4 Protein, necl-5 Protein, poliovirus receptor Protein, PVR Protein, Pvr Protein

Sujet: CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains. CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.

Poids moléculaire: 35.9 kDa

NCBI Accession: NP_006496

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome