DKK1 Protein (AA 32-266) (His tag)

N° du produit ABIN2180965

Détail du produit

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Type de proteíne: Recombinant

Attributs du protein: AA 32-266

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette DKK1 protéine est marqué à la His tag.

Séquence: AA 32-266

Attributs du produit: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.8 kDa. The protein migrates as 40-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Référence

Wu, Liu, Meng, Qu, Fu, Wang, Yang, Jin, Yu: "Neuropeptide Y enhances proliferation and prevents apoptosis in rat bone marrow stromal cells in association with activation of the Wnt/β-catenin pathway in vitro." dans: Stem cell research, Vol. 21, pp. 74-84, (2018) (PubMed).

Li, Kang, Sun, Liu, Wang, Gao: "The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling." dans: BioMed research international, Vol. 2015, pp. 345413, (2015) (PubMed).

Détail du antigène

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Autre désignation: Dkk-1 (DKK1 Produits)

Synonymes: DKK-1 Protein, SK Protein, AI414495 Protein, SGY Protein, SGY-1 Protein, SGY1 Protein, Soggy Protein, mSgy Protein, dkk1 Protein, dkk Protein, dkk-1 Protein, xdkk1 Protein, Xdkk-1 Protein, Dickkopf1 Protein, dickkopf-1 Protein, mdkk-1 Protein, dkk1-A Protein, dickkopf WNT signaling pathway inhibitor 1 Protein, dickkopf like acrosomal protein 1 Protein, dickkopf-related protein 1 Protein, dickkopf-like 1 Protein, dickkopf WNT signaling pathway inhibitor 1b Protein, dickkopf protein Protein, dickkopf WNT signaling pathway inhibitor 1 S homeolog Protein, DKK1 Protein, Dkk1 Protein, DKKL1 Protein, LOC100228789 Protein, Dkkl1 Protein, dkk1b Protein, dkk1 Protein, dickkopf Protein, dkk1.S Protein

Sujet: Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.

Poids moléculaire: 26.6 kDa

NCBI Accession: NP_036374

Pathways: Signalisation WNT, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation