WNT1 Protéine

N° du produit ABIN2469222

Détail du produit

Antigène: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Séquence: ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL

Attributs du produit: The ED50 was determined by its ability to enhance BMP - 2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/mL in the presence of 200 ng/mL of human BMP - 2.

Pureté: < 98 % by SDS-PAGE gel and HPLC analyses.

niveau d'endotoxine: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Conseil sur la manipulation: As with any protein, exposing Wnt-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Stock: -20 °C

Stockage commentaire: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Wnt-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Date de péremption: 24 months

Détail du antigène

Antigène: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Autre désignation: Wnt-1 (WNT1 Produits)

Synonymes: Xint-1 Protein, Xwnt1 Protein, int-1 Protein, int1 Protein, wnt-1 Protein, wnt1-a Protein, Int-1 Protein, Wnt-1 Protein, sw Protein, swaying Protein, BMND16 Protein, INT1 Protein, OI15 Protein, Int1 Protein, WNT-1 Protein, sb:eu647 Protein, zgc:194464 Protein, zgc:194478 Protein, WNT1 Protein, Wnt family member 1 Protein, Wnt family member 1 L homeolog Protein, wingless-type MMTV integration site family, member 1 Protein, WNT1 Protein, wnt1.L Protein, Wnt1 Protein, wnt1 Protein

Sujet: Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.

ID gène: 7471

NCBI Accession: NP_005421

OMIM: 4885655

UniProt: P04628

Pathways: Signalisation WNT, Dopaminergic Neurogenesis