IL-27 Protein (AA 1-16, AA 19-228, AA 29-234)

N° du produit ABIN2666690

Détail du produit

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-16, AA 19-228, AA 29-234

Origine: Souris

Source: HEK-293 Cells

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Pureté: Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 = 5 - 10 ng/ml, corresponding to a specific activity of 1.0 - 2.0 x 105 units/mg, as determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 20 mM NaH2PO4, pH 6, 0.60 M NaCl.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Autre désignation: IL-27 (IL27 Produits)

Synonymes: IL-27 Protein, IL-27A Protein, IL27A Protein, IL27p28 Protein, IL30 Protein, p28 Protein, RGD1561420 Protein, IL27 Protein, IL-27p28 Protein, Il30 Protein, interleukin 27 Protein, IL27 Protein, Il27 Protein

Sujet: IL-27 is a heterodimeric cytokine that consists of EBI3, an IL-12p40-related protein, and p28, an IL-12p35-related polypeptide. IL-27 can be allocated to the IL-6/IL-12 superfamily of cytokines which include also IL-23. This family is defined based on similarities in the structural motifs of the ligands, such as a common four-helix bundle, their receptors, which contain a hematopoietin receptor domain. IL-27 signaling occurs via a receptor complex composed of the signal transducing receptor chains WSX-1 and glycoprotein (gp) 130. Whereas WSX-1 is the IL-27-specific receptor chain, gp130 is the common receptor subunit of IL-6 type cytokines. IL-27 signaling in naive CD4+ T cells leads to activation and phosphorylation of STAT1 and STAT3.

Poids moléculaire: The total predicted molecular weight is 49 kDa. The non-reduced and the DTT-reduced proteins migrate as one band at approximately 55 kDa by SDS-PAGE.