TGFB1
Origine: Rat
Hôte: Escherichia coli (E. coli)
Recombinant
Greater than 95 % as determined by SDS-PAGE
WB, ELISA
Indications d'application
Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.
Buffer
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA.
Conseil sur la manipulation
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Stock
4 °C
Stockage commentaire
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.
Transforming growth factor beta 1 (TGF-β1) is a member of the transforming growth factor beta superfamily of cytokines. The TGF-β1 precursor contains 390 amino acids with an N-terminal signal peptide of 29 amino acids required for secretion from a cell, a 249 amino acid pro-region (latency associated peptide or LAP), and a 112 amino acid C-terminal region that becomes the active TGF-β1 upon activation. Both LAP and TGF-β1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF-β1 remain non-covalently associated, forming the small latent TGF-β complex (SLC, 100 kD). The large latent TGF-β complex (LLC, 235-260 kD) contains a third component, the latent TGF-β binding protein (LTBP), which is linked to LAP by a single disulfide bond. LTBP does not confer latency but is for efficient secretion of the complex to extracellular sites. Free active TGF-β1 can be released (activated) by many factors, including enzymes and low or high pH . TGF-β1 is nearly 100 % conserved across mammalian species. It has diverse biological functions in multiple cellular processes such as regulating proliferation and differentiation of various cell types. TGF-β1 is also an important immunoregulatory cytokine, which is involved in the maintenance of self-tolerance, Th17 differentiation, and T-cell homeostasis.
Poids moléculaire
Recombinant human TGF-β1 exists as a disulfide linked homodimer, consisting of two 112 amino acid monomers, each with a predicted molecular mass of approximately 12.8 kDa. The non-reduced protein migrates as a homodimer, at approximately 26 kDa by SDS-PAG