FGF8 Protein (AA 23-215, N-Term)

N° du produit ABIN2667402

Détail du produit

Antigène: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 23-215, N-Term

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Pureté: >97 % , as determined by Coomassie stained SDS-PAGE gel and HLPC analysis.

niveau d'endotoxine:

Less than 0.1 ng per μg of protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 = 2.0 - 4.0 ng/ml, corresponding to a specific activity of 2.5 - 5.0 x 105 units/mg,as measured by its ability to stimulate proliferation of BALB/c 3T3 cells in a dose dependent manner.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored at -20°C or -70°C.

Détail du antigène

Antigène: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Autre désignation: FGF-8 (FGF8 Produits)

Synonymes: Aigf Protein, Fgf-8 Protein, AIGF Protein, FGF-8 Protein, HBGF-8 Protein, HH6 Protein, KAL6 Protein, fibroblast growth factor 8 Protein, Fgf8 Protein, FGF8 Protein

Sujet: FGF-8 belongs to the fibroblast growth factor (FGF) family and plays an important role in cell growth, embryogenesis, and tumorigenesis. Four isoforms (a, b, e, and f) of human FGF-8 generated by alternative splicing of the mRNA have been described. The isoforms may have different functions during embryogenesis. During prenatal stages, FGF-8 is required for the normal development of various organs, including limbs and central nervous system. FGF-8 was first cloned from SC-3 mouse mammary carcinoma cells and was found to be induced in response to androgenic stimuli. FGF-8 is found to have a transforming capacity and is detected in human prostate and breast carcinoma specimens and cell lines. The isoform FGF-8b has the highest affinity to FGF receptors and the strongest transforming capacity.

Poids moléculaire: The 194 amino acid recombinant protein has a predicted molecular mass of approximately 22.5 kDa. The predicted N-terminal amino acid is Met.

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis