Interleukin 17a Protein (AA 20-155)

N° du produit ABIN2667465

Détail du produit

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 20-155

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Pureté: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

niveau d'endotoxine:

Less than 0.1 ng per μg of protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5.0 x 105 units/mg as determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored at -20°C or -70°C.

Détail du antigène

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Autre désignation: IL-17A (IL17A Produits)

Synonymes: CTLA8 Protein, IL-17 Protein, IL-17A Protein, IL17 Protein, Ctla-8 Protein, Ctla8 Protein, Il17 Protein, ChIL-17 Protein, IL-17F Protein, IL17A Protein, CTLA-8 Protein, interleukin 17A Protein, IL17A Protein, Il17a Protein

Sujet: IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8, It also shares 58 % homology with an open reading frame of the T-lymphotropic Herpesvirus Samirii virus (viral IL-17). IL-17A belongs to a family of cytokines, which has six members, designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4+ T cells (Th17) that develop in response to IL-23, in particular under conditions in which Th1 and Th2 development are suppressed. IL-17A shares the greatest homology (55 %) with IL-17F. Both IL-17A and IL-17F are produced by Th17 cells. IL-17A and IL-17F can either exist as IL-17A homodimers and IL-17F homodimers or as IL-17A-IL-17F heterodimers. IL-17 is a key mediator of autoimmune disorders, including rheumatoid arthritis, psoriasis, inflammatory bowel disease, and asthma, and plays a role in host defense.

Poids moléculaire: The 137 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15.7 kDa. The predicted N-terminal amino acid is Met. This protein exists as a disulfide-linked homodimer.