TGFA Protein (AA 40-89)

N° du produit ABIN2667579

Détail du produit

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 40-89

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Immunofluorescence (IF)

Pureté: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

niveau d'endotoxine:

Less than 0.1 ng per μg of protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg, as determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored at -20°C or -70°C.

Détail du antigène

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF-alpha (TGFA Produits)

Synonymes: TFGA Protein, LOC100009482 Protein, TGF alpha Protein, TGFA Protein, wa-1 Protein, wa1 Protein, RATTGFAA Protein, TGFAA Protein, NV15606 Protein, si:ch211-10e8.7 Protein, transforming growth factor alpha Protein, transforming growth factor alpha L homeolog Protein, transforming growth factor-alpha Protein, transforming growth factor, alpha Protein, TGFA Protein, TGF-A Protein, Tgfa Protein, tgfa.L Protein, LOC100121300 Protein, LOC100009150 Protein, tgfa Protein

Sujet: TGF-α was initially identified in the culture medium of several transformed cell lines. TGF-α is structurally and functionally related to the epidermal growth factor and belongs to the EGF subfamily that also includes amphiregulin (AR), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin (EPR), and epigen (EPG). TGF-α is derived from a transmembrane precursor (20-22 kD), pro-TGFα undergoes several posttranslational modifications that include N- and O-linked glycosylation and palmitoylation. The pro-TGFα includes a glycosylated N-terminal domain, a TGF-α region with the EGF-like motif, a linker sequence that joins the ectodomain to the transmembrane domain, and an intracellular portion. TGF-α is proteolytically released from the membrane by tumor necrosis factor-α converting enzyme (TACE/ADAM-17). TGF-α is cleaved at the N- and C-terminus of the EGF motif, the proteolytic cleavage at the C-terminus is required to release the soluble form. The N-terminal cleavage of pro-TGF α occurs at the cell surface by TACE-independent activity. TGF-α plays a key role regulating follicle development and tumorigenesis. TGF-α and its receptor are highly expressed in papillary thyroid carcinoma. Also, TGF-α polymorphism has been associated with oral birth defects.

Poids moléculaire: The 55 amino acid recombinant protein has a predicted Molecular mass of 5.5 kDa. The predicted N-terminal amino acid is Val.

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway