WNT7A Protein (AA 32-349)

N° du produit ABIN2667612

Détail du produit

Antigène: WNT7A (Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 32-349

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Multiplex Assay (MA)

Pureté: >80 % by SDS-PAGE gel and HPLC analyses.

niveau d'endotoxine:

Less than 0.1 ng per μg of protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: The ED50 was determined by its ability to inhibit WNT-3a induced alkaline phosphatase production in MC3T3-E1 cells. The estimated ED50 is 40-60 ng/ml, corresponding to a specific activity of 1.67 - 2.5 x 104 units/mg.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of ≤0.2 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored at -20°C or -70°C.

Détail du antigène

Antigène: WNT7A (Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A))

Autre désignation: WNT-7a (WNT7A Produits)

Synonymes: wnt7a Protein, AI849442 Protein, Wnt-7a Protein, px Protein, tw Protein, Xwnt-7a Protein, wnt-7a Protein, wnt7a-A Protein, Wnt family member 7A Protein, wingless-type MMTV integration site family, member 7Aa Protein, wingless-type MMTV integration site family, member 7A Protein, wingless-type MMTV integration site family member 7A S homeolog Protein, WNT7A Protein, wnt7aa Protein, Wnt7a Protein, wnt7a.S Protein

Sujet: The WNT protein family has a highly conserved cystein-rich structure, associated with the extracellular matrix (ECM) with high affinity through binding to heparin sulfate glycoproteins. They are carried intercellularly by lipoproteins and mediate signaling activity by binding to transmembrane Frizzled receptors and low-density-related proteins (LRP). They elicit a variety of responses through canonical or non-canonical (PCP or Ca++) pathways. There are two subtypes of WNT-7 that have been identified, WNT-7a and WNT-7b. WNT-7a is a secreted cell surface signaling protein that controls a set of pathways that act on cell fate determination and tissue patterning during embryogenesis. It is known that WNT-7a can modulate the development of the uterus, cerebellum and limbs, while WNT-7b is important in lung and placental development. The human amino acid sequence of WNT-7a shares high similarity (99.4 % ) with mouse WNT-7a. Signals transduced through WNT-7a and its receptor are found to be critical for female organ development, maintaining levels of female sex hormones and the development of oviduct and uterus. Receptors for WNT-7a include frizzled-5, frizzled-7 and frizzled-10. WNT-7a binds to frizzled-7 to induce signaling through SKT/ mTOR pathway in order to further modulate myofibers in a G-protein dependent PI3K pathway. During muscle cell regeneration, WNT-7a in the ECM along with transiently raised fibronectin levels in the stem cell niche can bind the frizzled-7/Sdc4 receptor complex and synergistically induce symmetric divisions of satellite stem cells. Also, in neurogenesis and chondrogenesis, WNT-7a contributes both in regulating and maintaining the level of lineage differentiation factors required to support apoptosis / cell death through binding of different receptors and interactions with ECM proteins.

Poids moléculaire: The 318 amino acids recombinant protein has a predicted molecular mass 35.5 kDa. The predicted N-terminal amino acid is Leu.

Pathways: Signalisation WNT, Stem Cell Maintenance, Asymmetric Protein Localization