Lactate Dehydrogenase A Protein (LDHA) (Transcript Variant 1) (Myc-DYKDDDDK Tag)

N° du produit ABIN2724571

Détail du produit

Antigène: Lactate Dehydrogenase A (LDHA)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Transcript Variant 1

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette Lactate Dehydrogenase A protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Functional Studies (Func), Standard (STD), Protein Interaction (PI)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human LDHA (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: Higher specific activity than endogenous human LDHA: OriGene human recombinant LDHA was compared side-by-side with purified human liver LDH5 in a spectrophotometric pyruvate to lactate conversion assay. Activity is shown as a decrease in absorbance at 340nm over time. The activity of recombinant human LDHA is comparable to that of endogenously expressed human LDH5.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: Lactate Dehydrogenase A (LDHA)

Autre désignation: Ldha (LDHA Produits)

Synonymes: GSD11 Protein, LDH1 Protein, LDHM Protein, LDH-M Protein, ldh-h Protein, ldha Protein, ldha-B Protein, ldhbb Protein, trg-5 Protein, Ldh1 Protein, Ldhm Protein, l7R2 Protein, gsd11 Protein, ldh-m Protein, ldh1 Protein, ldhm Protein, pig19 Protein, ldha1 Protein, ldhab Protein, LDH-A Protein, LDHC Protein, lactate dehydrogenase A Protein, lactate dehydrogenase B L homeolog Protein, lactate dehydrogenase A4 Protein, lactate dehydrogenase A L homeolog Protein, L-lactate dehydrogenase A chain Protein, LDHA Protein, ldhb.L Protein, Ldha Protein, ldha Protein, ldha.L Protein, LOC100713875 Protein

Sujet: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Poids moléculaire: 36.5 kDa

NCBI Accession: NP_005557

Pathways: L'effet Warburg