TIGAR Protein (TAT tag)
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- Antigène Voir toutes TIGAR Protéines
- TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette TIGAR protéine est marqué à la TAT tag.
- Application
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Specificité
- Optimal preservation of protein structure, post-translational modifications and functions.
- Attributs du produit
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- Recombinant human TIGAR protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Pureté
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- niveau d'endotoxine
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Pretreatment with TIGAR-TAT for 4 hrs, using a concentration range 0.1-5.0 ug/mL, protects U2OS cells from apoptosis induced by hydrogen peroxide.
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- Discover our top product TIGAR Protéine
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TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 2-270) protein (His tag)
TIGAR Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (GST tag)TIGAR Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (Strep Tag)Crystallography grade TIGAR Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-269) protein (Strep Tag)Crystallography grade TIGAR Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) protein (Myc-DYKDDDDK Tag)TIGAR Origine: Humain Hôte: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Indications d'application
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Conseil sur la manipulation
- Resuspend the protein in the desired concentration in proper buffer
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Antigène
- TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))
- Autre désignation
- Tigar (TIGAR Produits)
- Synonymes
- AA793651 Protein, AI595337 Protein, C79710 Protein, C85509 Protein, Tigar Protein, Trp53 induced glycolysis repulatory phosphatase Protein, Tigar Protein
- Sujet
- This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.
- Poids moléculaire
- 31.6 kDa
- NCBI Accession
- NP_065108
- Pathways
- L'effet Warburg
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